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KMID : 0363320120330040418
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Journal of Korean Oriental Internal Medicine
2012 Volume.33 No. 4 p.418 ~ p.428
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Effects of water and ethanol extracts from Ojeok-san on inflammation and its related diseases
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Yoo Sae-Room
Jeong Soo-Jin
Kim Ye-Ji
Lim Hye-Sun
Jin Seong-Eun
Jeon Woo-Young
Shin In-Sik
Shin Na-Ra
Kim Seong-Sil
Kim Jung-Hoon
Ha Hye-Kyung
Lee Mee-Young
Kim Ohn-Soon
Seo Chang-Seob
Shin Hyeun-Kyoo
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Abstract
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Objectives : Ojeok-san, a traditional herbal formula, has been used for the treatment of cold illness and its related
symptoms such as headache, nausea and indigestion. This study was performed to compare effects of water (OJSW) and 70% ethanol extracts (OJSE) of Ojeok-san on inflammation and its related diseases atopy, asthma and obesity in vitro.
Methods : We performed HPLC to investigate contents of index components of OJSW and OJSE. We investigated the effects
of OJSW and OJSE with an in vitro model, using 5 cell lines, specifically RAW 264. 7, HaCaT, MC/9, BEAS-2B and 3T3-L1.
Results : HPLC analysis displayed that the contents of index components were higher in OJSE than OJSW. In lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, OJSE significantly inhibited productions of interleukin (IL)-6, nitrite and prostaglandin E2 (PEG2). In TNF-α/IFN-γ-treated HaCaT keratinocytes, OJSE significantly lowered levels of macrophage-derived chemokine (MDC) as well as regulated and normal T cell expressed and secreted (RANTES). OJSE also had a protective effect on inflammatory response by decreasing RANTES secretion in TNF-α-stimulated BEAS-2B cells.
Conclusions : We conclude that OJSE could be more appropriate to enhance the biological activities against inflammation
and its related diseases, and could be applied as a bioactive material for developing the potent anti-inflammatory agents.
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KEYWORD
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Ojeok-san (Wuji-san), water extract, 70% ethanol extract, anti-inflammation, anti-allergic disease
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